Gamborg B5 medium
Description
Nitrate was required at a concentration of 20–30 mM for optimal growth. The addition of 2 mM ammonium sulfate enhanced cell growth; however, when supplied as the sole nitrogen source, NH₄⁺ alone failed to support proliferation. Similar outcomes were observed when NH₄NO₃ was used in place of (NH₄)₂SO₄. Notably, ammonium ions suppressed growth at concentrations exceeding 2 mM. Variations in phosphate, calcium, and magnesium levels induced only relatively minor changes in the growth rate. Thiamine, an essential nutrient, was found to improve cell growth at concentrations up to 10 mg/L.
Technical info
Components (per liter)
Macroelements
- CaCl₂ — 125.33 mg/L (1.13 mM)
- KH₂PO₄— 400 mg/L (2.94 mM)
- KNO₃ — 2830 mg/L (27.99 mM)
- MgSO₄ — 90.27 mg/L (0.75 mM)
- (NH₄)2NO₃ — 463 mg/L (3.50 mM)
Microelements
- FeNaEDTA — 36.70 mg/L (100 µM)
- H₃BO₃ — 1.60 mg/L (25.88 µM)
- MnSO₄·H₂O — 3.33 mg/L (19.70 µM)
- ZnSO₄·7H₂O — 1.50 mg/L (5.22 µM)
- KI — 0.80 mg/L (4.81 µM)
Storage Conditions
- Store dry at room temperature (15–25 °C).
- Keep tightly closed in original container.
- Protect from moisture and direct sunlight.
Hazard Statements (GHS classification)
- H315: Causes skin irritation.
- H319: Causes serious eye irritation.
- H335: May cause respiratory irritation.
Precautionary Statements
- P261: Avoid breathing dust/fume/gas/mist/vapors/spray.
- P305+P351+P338: IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses if present and easy to do. Continue rinsing.
- P302+P352: IF ON SKIN: Wash with plenty of water.
- P304+P340: IF INHALED: Remove person to fresh air and keep comfortable for breathing.
- P260: Do not breathe dust.
MSDS
MSDS
Certificate of Analysis
Certificate of Analysis
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