CHU (N6) Medium
Description
The concentration of ammonium was found to be a critical factor influencing callus development. The optimal NH₄⁺ concentration was determined to be 7.0 mM, which is equivalent to 3.5 mM of (NH₄)₂SO₄. Higher ammonium levels severely inhibited both growth and differentiation of rice pollen callus. In contrast, neither KNO₃ concentration nor other medium components had a significant effect on callus development. Chu's N6 medium was specifically designed to enhance the formation, growth, and differentiation of pollen-derived callus in rice.
Technical info
Components (per liter)
Macroelements
- CaCl₂ — 125.33 mg/L (1.13 mM)
- KH₂PO₄— 400 mg/L (2.94 mM)
- KNO₃ — 2830 mg/L (27.99 mM)
- MgSO₄ — 90.27 mg/L (0.75 mM)
- (NH₄)2NO₃ — 463 mg/L (3.50 mM)
Microelements
- FeNaEDTA — 36.70 mg/L (100 µM)
- H₃BO₃ — 1.60 mg/L (25.88 µM)
- MnSO₄·H₂O — 3.33 mg/L (19.70 µM)
- ZnSO₄·7H₂O — 1.50 mg/L (5.22 µM)
- KI — 0.80 mg/L (4.81 µM)
Storage Conditions
- Store dry at room temperature (15–25 °C).
- Keep tightly closed in original container.
- Protect from moisture and direct sunlight.
Hazard Statements (GHS classification)
- H315: Causes skin irritation.
- H319: Causes serious eye irritation.
- H335: May cause respiratory irritation.
Precautionary Statements
- P261: Avoid breathing dust/fume/gas/mist/vapors/spray.
- P305+P351+P338: IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses if present and easy to do. Continue rinsing.
- P302+P352: IF ON SKIN: Wash with plenty of water.
- P304+P340: IF INHALED: Remove person to fresh air and keep comfortable for breathing.
- P260: Do not breathe dust.
MSDS
MSDS
Certificate of Analysis
Certificate of Analysis
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